2 Primers and Pairs Select all amplicon markers present in your project. Configure primers and classifiers. Choose reference databases for each marker. 16S 16S rRNA Bacteria & Archaea TARGET REGION V3-V4 Other Forward Primer Reverse Primer REFERENCE DATABASE SILVA Custom Classifier Use your own trained QIIME2 classifier (.qza) Drop .qza file or click to browse 18S 18S rRNA Eukaryotes & Protists Coming Soon Forward Primer Reverse Primer REFERENCE DATABASE UNITE NCBI Custom Classifier Use your own trained QIIME2 classifier (.qza) Drop .qza file or click to browse ITS ITS1 / ITS2 Fungi & Mycobiome TARGET REGION ITS1 ITS2 Forward Primer Reverse Primer REFERENCE DATABASE UNITE Custom Classifier Use your own trained QIIME2 classifier (.qza) Drop .qza file or click to browse You can run multiple markers in the same analysis. Each individual sample can only be assigned to a single marker.
3 Sequencing Adapters Enter the adapter sequences for both read directions. These are applied uniformly to all samples in this run. Read 1 Adapter Read 2 Adapter
4 Samples Add your samples and import their FASTQ files. Each sample needs a Read 1 file; Read 2 is optional for paired-end. Drop a ZIP file with FASTQ files to auto-import FASTQ files must be inside primer subfolders and .zip ! No samples yet. Drop .zip with FASTQ files above. Please see section 4 of Guide on how to format directory tree and organize FASTQ files for seamless import.
5 Email Provide an email address to receive status updates and final results for this analysis. Email address We do not send marketing emails. You'll only receive updates related to this analysis. If your email is associated with an existing account, you'll be able to see this analysis on your dashboard.